Histochemical demonstration of phosphorylase in blood and bone marrow cells.

By TADAO rfAKEUCHI AND KENJI KIN0SHITA I N PREVIOUS STIJI)IES Takeuchi et al.’5 have showni that anssylophosphorylase could be demisonsstrated ins humisams amid ansimal tissue by histochemical nieanss. They did this by using the specific staimsimsg reactions of polysaccharide niewly fornssed frons glucose-i-phosphate as a result of emizynse activity. The presemsce of the enizyme was dennomistrated ins high comicenstrationss ins skeletal amid tonsgue nssuscles, heart muscle, snssooth muscles of various organss, brain cortex, sweat glansds, the squansous epithelium of t-otsgue, esophagus amid vagina, hair follicles amid cartilage cells. The enszynie was nsoderately amid weakly densonistrated in liver cells, collecting tubules of the kidney, glonseruli of fetal amid pigeons kidmseys, nsiammiiary glansd, cortical cells of the adrensal glansd, etc. Oms the other hanid, Rohdewald6 ins a recemst study reported that phosphorylase could be demisonsstrated 1)iochensically ins a suspensions of horse i)lood leukocytes. For this reasons we at-tenspted the histochensical detections of leukocyt-e phosphorylase ins blood cells. Although it was readily showms ott frowns sectionis ins the leukocytes of imiflansimnat-ory lesionss, it has heens difficult to demssomsstrate ims peripheral l)lOOd amid bonse misarrow smears. Recenitly, however, it was successfully densonistrated by our originsal technic amid the nsiethod amid experinsienital results are reported in this paper.